​Identification of a Human Y Chromosome Specific DNA Probe and Their Clinical Application by Fluorescence in situ Hybridization Techniques. Ran Suk Ryu1,2, Sang Chan Lee2, Chae Sik Lee2, Jong Heung Kim2, Eun Koung Ryu1 Sea Hwan Sohn1*​ 

Fertility and Sterility 27:405-415, 2000

Identification of a Human Y Chromosome Specific DNA Probe and Their 

Clinical Application by Fluorescence in situ Hybridization Techniques 

Ran Suk Ryu1,2, Sang Chan Lee2, Chae Sik Lee2, Jong Heung Kim2,

Eun Koung Ryu1 Sea Hwan Sohn1*

Department of Animal Science & Biotechnology, Chinju National University1,

SaeWha Obstetrics and Gynecology2

Objectives: The present study was undertaken to synthesize a human Y 

chromosome specific probe and to confirm the usefulness of the probe for 

fluorescence in situ hybridization(FISH) in various types of human cells. 

Methods: An approximately 400bp DNA fragment of the DYZ1 sequences was 

synthesized by PCR using digoxigenin labeled dUTP(dig-PCR). The fidelity 

of probe was tested by FISH for cultured and uncultured human lymphocytes, 

amniocytes, chorionic villus cells, embryos, sperms, and germ cells of 

seminiferous tubule.

Results: The human Y chromosome specific probe hybridized specifically to 

Y chromosome of the cells that had been tested. This probe assigned to the 

Yq12 region where the DYZ1 repetitive sequence is concentrated. 

Conclusions: We have identified a human Y chromosome specific probe that 

hybridized specifically to the Y chromosome by FISH for various types of 

uncultured as well as cultured cells. Therefore FISH technique using human 

Y chromosome specific probe should be useful for clinical application as a 

diagnostic tool for the detection of human Y chromosome. 

Key Words: FISH, DYZ1, Y chromosome, Amniocytes, Sperms