​Pronuclei Formation and Early Development of Human Oocytes after Intracytoplasmic Sperm Injection according to Maturity and Motility of Spermatozoa Geun-Joo Kim, Jong-Heung Kim, Sang-Chan Lee, and Byung-Ki Kim​ 

Dev. Reprod. Vol. 3, No. 1, 39-44, 1999

Pronuclei Formation and Early Development of Human Oocytes afrer 

Intracytoplasmic Sperm Injection according to Maturity an Motility of 

Spermatozoa

Geun-Joo Kim, Jong-Heung Kim*, Sang-Chan Lee* and Byung-Ki Kim

Department of biology, college of Natural Science, Dongeui University, 

Pusan, 614-714, Korea

*Saewha Infertility Institute, Pusan, Korea

ABSTRACT

In the present study, we investigated the effect of maturity of 

spermatozoa on the formation of pronucleus and subsequent developmental 

capacity of human embryo in vitro. The fertilization was perfored by means 

of intracytoplasmic sperm injection (ICSI) in HEPES-buffered m-TCM-199 

medium. In the first of the experiment, motile or immotile human 

spermatozoa ejaculated were injected into cumulus-enclosed human oocyte 

matured in vivo. Significantly(p<0.002) higher proportion of oocytes that 

was injected with motile spermatozoa formed 2 pronuclei than the oocytes 

injected with immotile spermatozoa (79.8% vs 51.7%). In the second part of 

the experiment, cumulus-enclosed human oocytes maturd in vivo were 

injected with motile or immotile spermatozoa collected form testes. There 

was no difference between motile and immotile spermatozoa. In third part 

of the experiment, using modified Tyrode's medium containing 10.0 mM 

lactate, 0.5mM pyruvate, 0.2 mM taurin, 1.0mM glutamine, 2.22 mM MEM amino 

acids, vitimin and 10% human follicular fluid, we found that the 

development of oocytes that formed 2 pronuclei were able to develop to 9-

16 cells regardless of maturity and motility of spermatozoa.

key words: Intracytoplasmic sperm injection, Ejaculated spermatozoa, 

Testicular spermatozoa, Pronuclei, In vitro development.